EDVOTEK Transformation of E-Coli (GFP) Kit
EDVOTEK Transformation of E-Coli (GFP) Kit
Product code: E8R05995
Product Description
In this Transformation of E-Coli Kit, students will explore the biological process of bacterial transformation using E-Coli and green fluorescent protein plasmid DNA.
At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
- Group Size: for 10 lab groups
- Storage: some components require refrigerator and freezer storage upon receipt
- Time Required: Set-up - 50 minutes, Incubation - overnight, Transformation - 15 minutes
The kit includes:
- Full instructions
- BactoBeads™ E.coli GFP Host
- Supercoiled pFluoroGreen™ plasmid DNA
- Ampicillin
- IPTG
- Calcium Chloride
- Growth Additive
- ReadyPour™ Luria Broth Agar (sterile)
- Luria Broth Medium for Recovery (sterile)
- Petri Plates (small & large)
- Plastic micro-tipped transfer pipettes
- Wrapped 10ml pipette (sterile)
- Toothpicks (sterile)
- Inoculating loops (sterile)
- Microcentrifuge tubes
Additional required equipment & materials (not supplied with the kit):
- Adjustable Micropipette (B8R05604)
- Micropipette Tips (PP00052738)
- 2 x Water Bath (B8R06048)
- Thermometer (B8A86150)
- Incubator (B8A64397)
- Pipette Filler (B8A78062)
- Ice
- Marker Pens (B8R08171)
- Bunsen Burner
- Microwave or Hot Plate (B8R04379)
- Hot gloves
- Long wave UV light
Please note - this product is non returnable.
- Explore genetic engineering and the central dogma with this elegant experiment
- Transform E.coli with pFluoroGreen™ - a plasmid containing genes for ampicillin resistance and the green fluorescent protein
- Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
- Expose transformed cells to IPTG to demonstrate differential gene expression
- New transformation protocol has high transformation efficiency = great results!
- A-Level Biology - Microbiology, Genetics, Transformation of E-Coli
- Full supporting materials included.
- EDVOTEK YouTube Channel is full of useful resources.
Further Information
- Age Recommended from
- 16 Years
- Brand
- EDVOTEK
- Culture Type
- E-Coli Green
- Kit Type
- Transformation of E-Coli Kit
- Number of Lab Groups per Kit
- 10 lab groups
- Product Type
- Bacterial Transformation Kit
- Storage
- Some components require refrigerator and freezer storage upon receipt
- Time Required
- Set-up - 50 minutesIncubation - overnightTransformation - 15 minutes
Documents
Useful Links
In this Transformation of E-Coli Kit, students will explore the biological process of bacterial transformation using E-Coli and green fluorescent protein plasmid DNA.
At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells.
- Group Size: for 10 lab groups
- Storage: some components require refrigerator and freezer storage upon receipt
- Time Required: Set-up - 50 minutes, Incubation - overnight, Transformation - 15 minutes
The kit includes:
- Full instructions
- BactoBeads™ E.coli GFP Host
- Supercoiled pFluoroGreen™ plasmid DNA
- Ampicillin
- IPTG
- Calcium Chloride
- Growth Additive
- ReadyPour™ Luria Broth Agar (sterile)
- Luria Broth Medium for Recovery (sterile)
- Petri Plates (small & large)
- Plastic micro-tipped transfer pipettes
- Wrapped 10ml pipette (sterile)
- Toothpicks (sterile)
- Inoculating loops (sterile)
- Microcentrifuge tubes
Additional required equipment & materials (not supplied with the kit):
- Adjustable Micropipette (B8R05604)
- Micropipette Tips (PP00052738)
- 2 x Water Bath (B8R06048)
- Thermometer (B8A86150)
- Incubator (B8A64397)
- Pipette Filler (B8A78062)
- Ice
- Marker Pens (B8R08171)
- Bunsen Burner
- Microwave or Hot Plate (B8R04379)
- Hot gloves
- Long wave UV light
Please note - this product is non returnable.
- Explore genetic engineering and the central dogma with this elegant experiment
- Transform E.coli with pFluoroGreen™ - a plasmid containing genes for ampicillin resistance and the green fluorescent protein
- Select for transformed cells using LB-ampicillin plates and calculate transformation efficiency
- Expose transformed cells to IPTG to demonstrate differential gene expression
- New transformation protocol has high transformation efficiency = great results!
- A-Level Biology - Microbiology, Genetics, Transformation of E-Coli
- Full supporting materials included.
- EDVOTEK YouTube Channel is full of useful resources.
Further Information
- Age Recommended from
- 16 Years
- Brand
- EDVOTEK
- Culture Type
- E-Coli Green
- Kit Type
- Transformation of E-Coli Kit
- Number of Lab Groups per Kit
- 10 lab groups
- Product Type
- Bacterial Transformation Kit
- Storage
- Some components require refrigerator and freezer storage upon receipt
- Time Required
- Set-up - 50 minutesIncubation - overnightTransformation - 15 minutes
